Thérapie cellulaire cardiaque avec cellules souches mésenchymateuses médullaires autologues : étude de la bio-disponibilité et la bio-distribution du greffon cellulaire

Abstract : Myocardial infarction (Mt) results in an apparent loss of contractile cardiomyocytes, a diminution of cardiac vessels (angiogenesis) which are responsible for the progressive alteration in the cardiac architecture and function. Recently, the reconstruction of the damaged cardiac tissue by using autologous stem cells (cardiac cell therapy)based partly on their ability to give rise to new cardiomyocytes and/or to promote angiogenesis has been proposed as a new therapeutic approach of the cardiac ceil therapy. This thesis describes different steps of pre-clinical investigation in order to assess the capability of bone marrow derived mesenchymal stem cells (BMSCs) to repair infarct and necrotic myocardium. After initial phases of validation of various techniques of BMSCs' selection (cell harvesting, culture and phenotype characterization) and the development of a rat model of myocardial infarction by permanent coronary ligation, the main focus of this project was to develop new investigational methodologies ailowing to track non-invasively implanted therapeutic cells. Therefore, the number of engrafted cells, their relative distribution within infarct versus normal myocardiai tissue might be precisely determined and sequentially followed up.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2006_0243_LI.pdf
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https://hal.univ-lorraine.fr/tel-01775365
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Submitted on : Tuesday, April 24, 2018 - 3:29:39 PM
Last modification on : Wednesday, April 25, 2018 - 1:32:02 AM

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  • HAL Id : tel-01775365, version 1

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Yan Li. Thérapie cellulaire cardiaque avec cellules souches mésenchymateuses médullaires autologues : étude de la bio-disponibilité et la bio-distribution du greffon cellulaire. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN10243⟩. ⟨tel-01775365⟩

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