7-alkylguanines et leurs dérivés fluorescents comme biomarqueurs d'exposition aux agents alkylants

Abstract : Biological fluids, such as gastric juice, often contain alkylating genotoxic substances which may paly a role in the aetiology of human cancer. The major adducts formed in DNA by most alkylating agents are 7-alkyldeoxyguanosines. These addcuts are labile and 7-alkylguanines are released by heating or enzyme repair. We have explored the possibility of using a probe DNA method - calf thymus DNA is incubated with nitrite-treated gastric juice, recovered and heated to induce depurination. The 7-alkylguanines are detected by direct fluorescence after an immunoaffinity purification, but with low sensitivity. 7-alkylguanines react with phenylmalondialdehyde to give derivatives with good HPLC-fluorescence properties. This method increases the detection sensitivity of 7-alkylguanine
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Marie-José Durand-Thouand. 7-alkylguanines et leurs dérivés fluorescents comme biomarqueurs d'exposition aux agents alkylants. Cancer. Université Paul Verlaine - Metz, 1993. Français. ⟨NNT : 1993METZ008S⟩. ⟨tel-01775436⟩

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