Compartmentalization of class 1 integrons and IncP-1 plasmids in the Orne river (France), an aquatic ecosystem impacted by urban and industrial anthropogenic pressures

Abstract : Mobile genetic elements (MGEs) are genetic structures frequently associated to the dissemination of antibiotic resistance genes (ARGs). In this work, we used two of them as proxies, class 1 integrons and IncP-1 plasmids, to better understand (i) the possible fate of ARGs once released in a river ecosystem (Orne, France), as well as (ii) the effect of anthropogenic pressures on their persistence. From river water analyses, we could show that the two MGEs do not behave the same way. The entry of class 1 integrons in the river system appeared to be diffuse rather than punctual, while the abundance of IncP-1 plasmid is relatively stable along the river section studied (23 km) thus indicating a rather indigenous origin. Anthropic inputs such as wastewater treatment plant did not seem to affect the abundance of MGEs because a too high level of effluent dilution. Interestingly, MGE-bearing bacteria appeared to be enriched on suspended material, which is likely to serve as a vehicle to drive MGE-richer communities of bacteria toward the sediments. The analysis of two sediment cores clearly indicates that only the top layers displayed an elevated level of MGE-bearing bacteria. These abundances decrease in deeper layers where only localized zones display micro-reservoirs of elevated MGE abundances. For one sediment core at least, we could show that the relative abundance of MGE negatively correlates with pollutants such as lead or certain PAHs
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Magali de la Cruz Barrón. Compartmentalization of class 1 integrons and IncP-1 plasmids in the Orne river (France), an aquatic ecosystem impacted by urban and industrial anthropogenic pressures. Ecosystems. Université de Lorraine, 2018. English. ⟨NNT : 2018LORR0212⟩. ⟨tel-02096872⟩

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