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Étude fonctionnelle de facteurs de maturation tardifs des protéines fer-soufre chloroplastiques chez A. thaliana

Abstract : Numerous cellular processes such as respiration, photosynthesis, nitrogen and sulfur assimilation or vitamin and secondary metabolite synthesis, rely on iron-sulfur (Fe-S) proteins. In plants, the maturation of chloroplastic, mitochondrial and cytosolic or nuclear proteins depends on the SUF, ISC and CIA machineries respectively. In plastids, an Fe-S cluster is assembled on the SUFBC2D scaffold complex before being transferred to target proteins via transfer proteins. The objective was to understand the role of the SUFA1, IBA57.2 and NFU1 transfer proteins by analyzing their ability to bind Fe-S clusters and isolating their partners within the SUF machinery and among known chloroplastic targets in order to elucidate the molecular mechanisms of these interactions. In vitro Fe-S cluster reconstitution experiments using recombinant proteins have shown that SUFA1, NFU1 and the SUFA1-IBA57.2 complex bind different types of Fe-S clusters. In addition, NFU1 binds a [4Fe-4S] cluster within a dimer that can be transferred to SUFA1, but also to the target proteins, ISPG and THIC, two enzymes involved in the synthesis of isoprenoids and thiamine. All the interactions identified by yeast two-hybrid and/or BiFC for these proteins, but also for NFU2, NFU3 and HCF101, allowed refining their respective roles for the maturation of some of the 50 chloroplastic Fe-S proteins.
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Submitted on : Friday, April 24, 2020 - 3:10:40 PM
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Mélanie Roland. Étude fonctionnelle de facteurs de maturation tardifs des protéines fer-soufre chloroplastiques chez A. thaliana. Biologie végétale. Université de Lorraine, 2019. Français. ⟨NNT : 2019LORR0235⟩. ⟨tel-02553538⟩



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