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Nanofunctionalization and biofabrication of natural hydrogels for tissue engineering applications

Abstract : The main objective of this thesis is to develop a new natural material based on methacrylated gelatin (GelMA) nanofunctionalized by the incorporation of nanoliposomes or soft hybrid exosome-liposome nanoparticles. The physicochemical and biological properties of these hydrogel matrices were characterized in order to evaluate their potential use for tissue engineering applications. GelMA is prepared by the chemical modification of gelatin when methacrylate groups are attached to side groups containing amine functions. In a first part of this work, the influence of the gelatin source (pork or fish) and the degree of methacrylation on the physicochemical and biological properties of hydrogels was studied. In a second part of this work, the GelMA matrix was nanofunctionalized by the incorporation of nanoliposomes, which are soft and natural nanoparticles with remarkable self-assembly properties. These well-established drug delivery systems‎ are formed of lipid bilayers and can transport and release hydrophobic, hydrophilic, and amphiphilic molecules. In this study, naringin, an active molecule that can guide the differentiation process of stem cells to the osteoblastic lineage, was encapsulated in nanoliposomes before their incorporation into the GelMA polymeric matrix in order to develop a system of interest for bone regeneration applications. This nanocomposite material was physicochemically and biologically characterized and the release profile of naringin was investigated. In a third and final part of this work, the GelMA matrix was nanofunctionalized by the incorporation of exosome-liposome soft hybrid nanoparticles. Exosomes, natural nanovesicles secreted by cells, are of increasing interest for targeted drug delivery applications due to the presence of cell specific receptors on their surface. The hybrid GelMA hydrogels were physicochemically and biologically characterized for applications in cardiac reprogramming and was successfully bioprinted and microfabricated. Biofabricated GelMA hydrogels nanofunctionalized with nanoliposomes or hybrid exosome-liposome nanoparticles are promising platforms for the controlled release of bioactive molecules and for tissue engineering applications.
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https://hal.univ-lorraine.fr/tel-03285184
Contributor : Thèses Ul <>
Submitted on : Tuesday, July 13, 2021 - 10:47:03 AM
Last modification on : Monday, August 16, 2021 - 3:04:15 PM

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  • HAL Id : tel-03285184, version 1

Citation

Kamil Elkhoury. Nanofunctionalization and biofabrication of natural hydrogels for tissue engineering applications. Biotechnology. Université de Lorraine, 2021. English. ⟨NNT : 2021LORR0020⟩. ⟨tel-03285184⟩

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