Skip to Main content Skip to Navigation
Theses

Étude de l’interaction des norovirus avec les antigènes tissulaires de groupes sanguins humains au cours de l’inactivation virale

Abstract : Noroviruses (NoVs) are one of the leading causes of gastroenteritis worldwide. The detection of NoVs in food and water is performed by using molecular tools, but they do not provide information about NoV infectivity when their genome is detected. The recognition of the cellular receptor prior the detection of viral genome could limit the overestimation of the viral hazard by excluding non-infectious particles having altered capsid. Many studies have shown that the specific interaction between histo-blood group antigens (HBGA) and the viral capsid promotes the infection of NoVs in humans. These complex carbohydrates, found in saliva and on the surface of intestinal cells, are described as attachment factors but it is not excluded that they may play a role of cellular co-receptor or a role of protection of NoVs against the stresses found in the gastrointestinal tract before reaching its host cell. Firstly, inactivation factors that viruses can encountered before reaching the human intestine (i.e. acid pH, proteolytic enzymes) have been tested to evaluate the role of NoV protection by HBGAs. Secondly, various inactivation treatments (i.e. natural ageing, heat, oxidants) have been tested to evaluate the capacity of HBGA-binding to the GII.4 NoV capsids, and thus the capsid integrity. Using complementary methodological approaches, it was evidenced that the interaction between HBGAs and viral capsid did not protect GII.4 NoV against acid pH and proteolytic enzymes. Then, our results have demonstrated that the HBGA-binding to the capsid could select GII.4 NoVs having structured capsids. However, this approach displays some drawbacks for inactivation treatments which induce minor changes in the capsid integrity, suggesting that the loss of the HBGA-binding to the NoV capsid could not always be correlated to the loss of the NoV infectivity.
Document type :
Theses
Complete list of metadata

https://hal.univ-lorraine.fr/tel-03335496
Contributor : Thèses Ul <>
Submitted on : Monday, September 6, 2021 - 11:44:25 AM
Last modification on : Tuesday, September 7, 2021 - 3:18:37 AM

File

DDOC_T_2021_0030_CHASSAING.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-03335496, version 1

Citation

Manon Chassaing. Étude de l’interaction des norovirus avec les antigènes tissulaires de groupes sanguins humains au cours de l’inactivation virale. Virologie. Université de Lorraine, 2021. Français. ⟨NNT : 2021LORR0030⟩. ⟨tel-03335496⟩

Share

Metrics

Record views

33

Files downloads

4