Doxorubicin-loaded glycyrrhetinic acid modified recombinant human serum albumin nanoparticles for targeting liver tumor chemotherapy

Abstract : Due to overexpression of glycyrrhetinic acid (GA) receptor in liver cancer cells, glycyrrhetinic acid modified recombinant human serum albumin (rHSA) nanoparticles for targeting liver tumor cells may result in increased therapeutic efficacy and decreased adverse effects of cancer therapy. In this study, doxorubicin (DOX) loaded and glycyrrhetinic acid modified recombinant human serum albumin nanoparticles (DOX/GA-rHSA NPs) were prepared for targeting therapy for liver cancer. GA was covalently coupled to recombinant human serum albumin nanoparticles, which could efficiently deliver DOX into liver cancer cells. The resultant GA-rHSA NPs exhibited uniform spherical shape and high stability in plasma with fixed negative charge (∼-25 mV) and a size about 170 nm. DOX was loaded into GA-rHSA NPs with a maximal encapsulation efficiency of 75.8%. Moreover, the targeted NPs (DOX/GA-rHSA NPs) showed increased cytotoxic activity in liver tumor cells compared to the nontargeted NPs (DOX/rHSA NPs, DOX loaded recombinant human serum albumin nanoparticles without GA conjugating). The targeted NPs exhibited higher cellular uptake in a GA receptor-positive liver cancer cell line than nontargeted NPs as measured by both flow cytometry and confocal laser scanning microscopy. Biodistribution experiments showed that DOX/GA-rHSA NPs exhibited a much higher level of tumor accumulation than nontargeted NPs at 1 h after injection in hepatoma-bearing Balb/c mice. Therefore, the DOX/GA-rHSA NPs could be considered as an efficient nanoplatform for targeting drug delivery system for liver cancer.
Type de document :
Article dans une revue
Molecular Pharmaceutics, American Chemical Society, 2015, 12 (3), pp.675-683. 〈10.1021/mp500394v〉
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01281139
Contributeur : Cithefor Ul <>
Soumis le : mardi 1 mars 2016 - 16:34:48
Dernière modification le : mercredi 2 mars 2016 - 01:10:25

Identifiants

Collections

Citation

Wen-Wen Qi, Hai-Yan Yu, Hui Guo, Jun Lou, Zhi-Ming Wang, et al.. Doxorubicin-loaded glycyrrhetinic acid modified recombinant human serum albumin nanoparticles for targeting liver tumor chemotherapy. Molecular Pharmaceutics, American Chemical Society, 2015, 12 (3), pp.675-683. 〈10.1021/mp500394v〉. 〈hal-01281139〉

Partager

Métriques

Consultations de la notice

49