Hypergravity exposure during gestation modifies the TCRβ repertoire of newborn mice

Abstract : During spaceflight, organisms are subjected to mechanical force changes (gravity (G) changes) that affect the immune system. However, gravitational effects on lymphopoiesis have rarely been studied. Consequently, we investigated whether the TCR beta repertoire, created by V(D)J recombination during T lymphopoiesis, is affected by hypergravity exposure during murine development. To address this question, C57BL/6j mice were mated in a centrifuge so that embryonic development, birth and TCR beta rearrangements occurred at 2G. Pups were sacrificed at birth, and their thymus used to quantify transcripts coding for factors required for V(D) J recombination and T lymphopoiesis. We also created cDNA mini-libraries of TCR beta transcripts to study the impact of hypergravity on TCR beta diversity. Our data show that hypergravity exposure increases the transcription of TCR beta chains, and of genes whose products are involved in TCR signaling, and affects the V(D) J recombination process. We also observed that similar to 85% of the TCR beta repertoire is different between hypergravity and control pups. These data indicate that changing a mechanical force (the gravity) during ontogeny will likely affect host immunity because properties of loops constituting TCR antigen-binding sites are modified in hypergravity newborns. The spectrum of peptides recognized by TCR will therefore likely be different.
Type de document :
Article dans une revue
Scientific Reports, Nature Publishing Group, 2015, 5, pp.9318. 〈http://www.nature.com/articles/srep09318〉. 〈10.1038/srep09318〉
Liste complète des métadonnées

Littérature citée [43 références]  Voir  Masquer  Télécharger

https://hal.univ-lorraine.fr/hal-01481951
Contributeur : Simpa Ul <>
Soumis le : vendredi 3 mars 2017 - 10:05:59
Dernière modification le : mercredi 22 novembre 2017 - 11:02:23
Document(s) archivé(s) le : mardi 6 juin 2017 - 12:25:18

Fichier

srep09318.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Stéphanie Ghislin, Nassima Ouzren-Zarhloul, Sandra Kaminski, Jean-Pol Frippiat. Hypergravity exposure during gestation modifies the TCRβ repertoire of newborn mice. Scientific Reports, Nature Publishing Group, 2015, 5, pp.9318. 〈http://www.nature.com/articles/srep09318〉. 〈10.1038/srep09318〉. 〈hal-01481951〉

Partager

Métriques

Consultations de la notice

72

Téléchargements de fichiers

31