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In vivo response of some immune and endocrine variables to LPS in Eurasian perch (Perca fluviatilis, L.) and modulation of this response by two corticosteroids, cortisol and 11-deoxycorticosterone

Abstract : In fish, the endocrine system, especially corticosteroids pathway, strongly interacts with immune system. On the other hand, in vivo co-stimulation of both systems is not well documented. To better understand this interaction, we derided to evaluate the in vivo effects of both stimulation of the immune system and co-stimulation of both systems in Eurasian perch juveniles. Fish were injected either with 10 mg kg(-1) LPS, or with a combination of LPS and 0.8 mg kg(-1) cortisol or LPS and 0.08 mg kg(-1) 11-deoxycorticosterone (DOC) and sampled 1,3 or 7 days after injection. LPS affected the immune system by increasing plasma lysozyme activity and blood neutrophils populations. During the same time-course, LPS decreased the proportion of a mixture of lymphocytes and thrombocytes in blood and TNF-alpha expression in spleen. Cortisol modulated the LPS-mediated response in TNF-alpha mRNA expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated the abundance of complement component 3 (C3) mRNA in spleen. On the other hand, LPS altered the corticotropic axis by decreasing mRNA expression levels of all corticosteroid receptors and of 11 beta-HSD-2 in spleen. Both corticosteroids injected were not able to balance these LPS-induced suppressive effects on corticosteroid receptors and 11 beta-HSD-2 expression levels in spleen. Contrary to LPS alone, the association of LPS with DOC modulated GR-1b expression in gills. These results indicated that LPS is a strong modulator of the corticosteroid receptors expression in spleen. Furthermore, we report for the first time a LPS-induced decrease of the mineralocorticoid receptor expression. Finally, corticosteroids were able to modulate the LPS-mediated response at the transcriptional level.
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https://hal.univ-lorraine.fr/hal-01562166
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Submitted on : Thursday, July 13, 2017 - 4:35:43 PM
Last modification on : Friday, February 5, 2021 - 3:43:01 AM

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Cédric Mathieu, Sylvain Milla, S. N. M. Mandiki, Jessica Douxfils, Patrick Kestemont. In vivo response of some immune and endocrine variables to LPS in Eurasian perch (Perca fluviatilis, L.) and modulation of this response by two corticosteroids, cortisol and 11-deoxycorticosterone. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2014, 167, pp.25-34. ⟨10.1016/j.cbpa.2013.09.006⟩. ⟨hal-01562166⟩

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