Mise au point d’un système de purification de la molécule signal impliquée dans la communication entre les bactéries Streptomyces ambofaciens et Pseudomonas fluorescens

Abstract : Streptomyces ambofaciens ATCC23877 is a soil bacterium known to produce many secondary metabolites, some of which are of industrial interest. Interaction studies between S. ambofaciens and other soil bacteria have revealed an interaction with Pseudomonas fluorescens BBc6R8. P. fluorescens is able to influence the control of antibiotic synthesis in S. ambofaciens. In the presence of P. fluorescens, S. ambofaciens no longer produces kinamycin, an antibiotic synthesized by the alp cluster. The kinamycin biosynthesis genes are regulated in this cluster by a protein called AlpZ. This protein is capable of binding to specific sequences of DNA in the promoter regions of certain genes and to suppress their expression. However, a signal molecule close to the family of ?- butyrolactones can, when it is present in high enough quantities, bind to AlpZ and prevent its repressive effect. The unknown signal molecule, ligand of Alpz, is a candidate as a target of P. fluorescens. Thus, this work aims to make possible the purification of this unknown molecule for its characterization. Separately, a reporter system which allows to easily detect the presence of this molecule is constructed. It would thus lead to learning more about the interaction between these two bacteria, and about the model for regulating the synthesis of kinamycine.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/BUS_M_2014_MEDOT_JEAN-SELIM.pdf
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https://hal.univ-lorraine.fr/hal-01774220
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Submitted on : Monday, April 23, 2018 - 2:44:31 PM
Last modification on : Friday, September 14, 2018 - 1:13:09 AM

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Jean-Sélim Medot. Mise au point d’un système de purification de la molécule signal impliquée dans la communication entre les bactéries Streptomyces ambofaciens et Pseudomonas fluorescens. Microbiologie et Parasitologie. 2014. ⟨hal-01774220⟩

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