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Adenovirus-specific T-lymphocyte efficacy in the presence of methylprednisolone: An in vitro study

Abstract : Virus-specific T-cell (VST) infusion becomes a promising alternative treatment for refractory viral infections after hematopoietic stem cell transplantation (HSCT). However, VSTs are often infused during an immunosuppressive treatment course, especially corticosteroids, which are a first-line curative treatment of graft-versus-host disease (GVHD). We were interested in whether corticosteroids could affect adenovirus (ADV)-VST functions. After interferon (IFN)-γ based immunomagnetic selection, ADV-VSTs were in vitro expanded according to three different culture conditions: without methylprednisolone (MP; n = 7), with a final concentration of MP 1 µg/mL (n = 7) or MP 2 µg/mL (n = 7) during 28 ± 11 days. Efficacy and alloreactivity of expanded ADV-VSTs was controlled in vitro. MP transitorily inhibited ADV-VST early expansion. No impairment of specific IFN-γ secretion capacity and cytotoxicity of ADV-VSTs was observed in the presence of MP. However, specific proliferation and alloreactivity of ADV-VSTs were decreased in the presence of MP. Altogether, these results and the preliminary encouraging clinical experiences of co-administration of MP 1 mg/kg and ADV-VSTs will contribute to safe and efficient use of anti-viral immunotherapy.
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https://hal.univ-lorraine.fr/hal-01777722
Contributor : Maud d'Aveni <>
Submitted on : Wednesday, April 25, 2018 - 9:37:26 AM
Last modification on : Tuesday, October 13, 2020 - 10:47:44 AM

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Arnaud Campidelli, Chongsheng Qian, Caroline Laroye, Véronique Decot, Loïc Reppel, et al.. Adenovirus-specific T-lymphocyte efficacy in the presence of methylprednisolone: An in vitro study. Cytotherapy, Elsevier, 2018, 20 (4), pp.524 - 531. ⟨10.1016/j.jcyt.2017.12.010⟩. ⟨hal-01777722⟩

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