Recherche de nouveaux métabolites secondaires chez Streptomyces ambofaciens par une approche novatrice de génomique comparée

Abstract : Secondary metabolites (SM) are used since a long time in different domains like human and veterinary medicine (antibiotic, antitumor) or agriculture (anti-fungal). Streptomyces ambofaciens, StrAda team’s model strain, produces a large number of secondary metabolites including five antimicrobial molecules. A mutant strain unable to produce the spiramycin, congocidine, and kinamycin antibiotics was mutated independently in each of the other SM biosynthetic gene clusters identified by the sequencing of the S. ambofaciens genome. Despite these mutations, an antibacterial activity remains. That indicates that a new kind of biosynthetic genes cluster which cannot be detected by homology based approaches is responsible of this activity. The biosynthetic genes could be located in a genomic island (GI), the SM biosynthetic genes clusters being often found in GIs. The aim of my internship is to test this hypothesis by two approaches: the GI’s deletion by using the CRISPR system, and the GI’s heterologous expression, by using the TAR system, in a Streptomyces strain dedicated to SM production.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/public/BUS_M_2016_HOUIN_ANTHONY.pdf
Complete list of metadatas

Cited literature [74 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/hal-01824836
Contributor : Memoires Ul <>
Submitted on : Wednesday, June 27, 2018 - 4:15:42 PM
Last modification on : Thursday, June 28, 2018 - 3:09:31 PM
Long-term archiving on : Thursday, September 27, 2018 - 1:55:10 AM

File

BUS_M_2016_HOUIN_ANTHONY.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : hal-01824836, version 1

Collections

Citation

Anthony Houin. Recherche de nouveaux métabolites secondaires chez Streptomyces ambofaciens par une approche novatrice de génomique comparée. Biotechnologies. 2016. ⟨hal-01824836⟩

Share

Metrics

Record views

26

Files downloads

83