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Master thesis

Contribution à l'étude des facteurs de régulation transcriptionnelle impliqués dans la synthèse des effecteurs symbiotiques chez le champignon ectomycorhizien Laccaria bicolor

Abstract : Laccaria bicolor is an ectomycorrhizal fungus (ECM) which is able to make a mutualistic symbiosis with poplar. In exchange for carbohydrates, ECM fungi offer an improved mineral supply and stress protection for trees. So ECM play a major role in forestry ecosystem functionning. However the current knowledge about molecular mecanisms involved in symbiosis establishment has to be improved. We have studied the effect of plant diffusible molecules, rutin and quercetin (two root exudates) on biomasse, growth and morphology of Laccaria bicolor after 24h until 14 days of exposure. We were not able to detect any effect of rutin nor quercetin nor plant roots exudates on, biomass production, colonial growth and hyphal branching at the concentrations and exposure times tested. Moreover gene expression patterns of MiSSP7, MiSSP8,5 and MiSSP17 were also determined after 24h exposure of L.bicolor to rutin or quercetin or poplar roots by RTqPCR. We observed an upregulation of MiSSP7 after 24h exposure to poplar roots. Finally, data sets obtain during this internship contributes in understanding and modelling genes mecanisms involved in L. bicolor ECM development.
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Laura Weiss. Contribution à l'étude des facteurs de régulation transcriptionnelle impliqués dans la synthèse des effecteurs symbiotiques chez le champignon ectomycorhizien Laccaria bicolor. Microbiologie et Parasitologie. 2015. ⟨hal-01828871⟩

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