Pikeperch (Sander lucioperca) has a high potential for inland aquaculture diversification in Europe. Bottlenecks (i.e. low survival, cannibalism and deformity rates) hamper further expansion of pikeperch culture, because of the weak production performances at the nursery level. To improve the production of pikeperch juveniles under recirculation system we used a pilot scale larval rearing system (700 L tanks) and multifactorial designs. Three successive larval rearing trials (duration: 35-49 days) were conducted to identify the best combination of environmental, feeding and population factors. Considering the main significant effects observed on survival, growth, swim bladder inflation rates and biomass gain, a favourable combination of twelve factor (F) modalities was proposed (F1- initial density: 100 larvae.L-1, F2- no sorting of fish jumper, F3- no sibling population, F4- eggs from large females, F5- discontinuous feeding, F6- no co-feeding, F7- light intensity: 50 lx, F8- beginning of the weaning at 16 dph (days post-hatching), F9- weaning duration: 9 days, F10- water renewal rate of 100 % per hour, F11- tank cleaning during morning and F12- tank bottom-up water current). A final validation step was realized over a last trial (seven replicates, duration: 49 days), and validated with the best productive results obtained over the global experimental period (2015-2018). These results were: a final body weight of 815.64 +/- 95.34 mg, a survival rate of 16.9 +/- 1.7 %, a specific growth rate of 15.1 +/- 5.9 %.d(-1), a final fish biomass of 9.55 +/- 0.23 kg, a swim bladder inflation rate of 92.6 +/- 3.2 % and a food conversion rate of 0.65 +/- 0.02 (dry food). The final stocking density was 13.6 kg.m(-3) of rearing volume. Authors were able to validate and provide a reliable basic protocol for pikeperch larval rearing using recirculating units.