Desulfovibrio spp. survive within KB cells and modulate inflammatory responses - Archive ouverte HAL Access content directly
Journal Articles Molecular Oral Microbiology Year : 2010

Desulfovibrio spp. survive within KB cells and modulate inflammatory responses

(1) , (2) , (3) , (4) , (5)
1
2
3
4
5

Abstract

Desulfovibrio are sulfate-reducing anaerobic gram-negative rods that have been proposed as potential periodontopathogens. We investigated the capacity of Desulfovibrio to invade epithelial cells and induce cytokine secretion from these cells. Desulfovibrio strains were co-cultured with KB cells and counts of intracellular bacteria evaluated up to 3 days after infection. Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis were able to survive within epithelial cells. Intracytoplasmic location of both bacterial species was confirmed by confocal laser scanning microscopy and transmission electron microscopy. Invasion was sensitive to nocodazole, an inhibitor of microtubule polymerization, but not to cytochalasin D, a microfilament inhibitor, suggesting that microtubule rearrangements were involved in the internalization of Desulfovibrio strains by KB cells. Infection by Desulfovibrio resulted in increased production of IL-6 and IL-8 by KB cells. The ability of D. desulfuricans and D. fairfieldensis to survive within oral epithelial cells and to modulate the epithelial immune response may contribute to the initiation and progression of periodontal diseases.
Fichier principal
Vignette du fichier
Bisson-2010.DesuloSurviveKBCells&ModulateInflamResponses.pdf (342.99 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02987410 , version 1 (03-11-2020)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

C Bisson-Boutelliez, F. Massin, D. Dumas, N Miller, A. Lozniewski. Desulfovibrio spp. survive within KB cells and modulate inflammatory responses. Molecular Oral Microbiology, 2010, 25 (3), pp.226-35. ⟨10.1111/j.2041-1014.2009.00550.x⟩. ⟨hal-02987410⟩
42 View
166 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More