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CD9 and HLA-DR expression by crevicular epithelial cells and polymorphonuclear neutrophils in periodontal disease

Abstract : Background, aims: The composition of gingival crevicular fluid (GCF) is likely to reflect inflammatory modifications that take place in the gingiva during peri-odontal diseases. Method: In this study, GCF was collected at 3 different sites from 23 periodontal patients. The sites were assessed to be healthy, presenting gingivitis or periodontitis. 10 healthy individuals without any form of periodontal disease formed the control group and were sampled at one site each. The cell content of GCF was collected using Durapore Millipore strips, and 2 types of cells were studied: epi-thelial cells (EC) and polymorphonuclear neutrophils (PMN). The expression of CD9 and HLA-DR within or on the surface of these cells was studied in immunofluorescence on cytospin smears. Results: Both CD9 and HLA-DR expression on EC differed significantly from control subjects, and the latter decreased according to the severity of the pathology. None of the PMN found in controls expressed CD9 or HLA-DR. However, in periodontal patients, the expression of HLA-DR within PMNs was detectable and increased according to the severity of lesions. CD9 expression on PMNs also increased with inflammation. Conclusion: This study shows that clinically healthy sites of periodontal patients inflammation; crevicular polymorphonuclear already present signs of immunological activation characterised by a down neutrophils; epithelial cells; CD9; HLA-DR.
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https://hal.univ-lorraine.fr/hal-02988703
Contributor : Catherine Bisson Connect in order to contact the contributor
Submitted on : Wednesday, February 10, 2021 - 11:41:14 AM
Last modification on : Thursday, February 11, 2021 - 3:28:24 AM

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Catherine Bisson, N Miller, D Demarch, M Bene. CD9 and HLA-DR expression by crevicular epithelial cells and polymorphonuclear neutrophils in periodontal disease. Journal of Clinical Periodontology, Wiley, 2001, 28, pp.650 - 656. ⟨10.1034/j.1600-051x.2001.028007650.x⟩. ⟨hal-02988703⟩

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