We experimentally studied sequential {101-2} twinning in rolled pure titanium at room temperature and identified a new sequential twinning mechanism, i.e., {101-2} (T) extension twin stimulated by the twin-twin junction of two primary {1121} extension twins T and T, which holds a certain relation T T→T. Schmid factor analysis, as the local stress is taken into account, is able to determine the position of sequential twin variant, but cannot determine the twin variant. Displacement gradient accommodation is used to determine the twin variant. The well-known secondary {1012} (T) extension twins in primary {1122} (C) contraction twins (referred to as C → T double twinning) are also analyzed according to a generalized Schmid factor analysis. Displacement gradient accommodation and twin nucleation based on dislocations dissociation only work well for the most active twin variants, but cannot address other phenomena associated with C → T double twinning. For rarely activated twin variants, displacement gradient accommodation was not satisfied.