Twinning is a frequent deformation mechanism in nanocrystalline metals, and segregation of solute atoms at twin boundaries is a thermodynamic process that plays an important role in the stability and strengthening of these materials. In pristine, defect-free twin boundaries, solute segregation generally follows a single-or multi-layer patterned coverage of solutes that is uniformly and symmetrically distributed at segregation sites across the boundary. However, when a disconnection, a type of interfacial line defect, is present at the twin boundary, we report a possible discontinuity of the segregation patterns across this defect for a broad range of binary alloys. The change of segregation pattern is explained by a break of the local symmetry across the disconnection terraces. The characteristics of this change are dictated by the orientation of the dislocation content sitting at the step region of the disconnection and its synergistic/antagonistic interactions with the step character. These findings not only advance our understanding of the origin of the interface segregation phenomena and the key contribution from interfacial defects, but they also shed light on applications for tailoring atomically precise interfacial structures to design alloys with emerging properties.