Role of container materials on polycyclic aromatic compound (PAC) concentrations in aqueous phase: implication for aquatic ecotoxicity determination - Université de Lorraine Accéder directement au contenu
Poster Année : 2023

Role of container materials on polycyclic aromatic compound (PAC) concentrations in aqueous phase: implication for aquatic ecotoxicity determination

Résumé

In historically contaminated sites such as coking and manufactured gas plants, other families of organic pollutants than the 16 regulated PAHs can occur. Polar polycyclic aromatic compounds (PACs), are also present in the initial pollution and can also be generated over time. Their aqueous solubilities are much greater than those of the PAHs, and this may induce the contamination of groundwater resources. It is though important to evaluate the potential risk associated with the occurrence of PACs in body waters. A classical approach is to run standard aquatic bioassay using model organisms, such as rotifer (Brachionus calyciflorus) (ISO 20666, 2009) and green algae (Pseudokirchneriella subcapitata) (ISO 8692, 2012) to evaluate the associated ecotoxicity. The objective of this study is to determine the fate of PACs during standard in-vivo aquatic ecotoxicity testing. Indeed, rotifers and algae ecotoxicity testing are usually carried out in polystyrene (PS) 24 and 96 wells microplates, respectively, and little is known about the PAC sorption capacity to PS under test conditions. However, this information is crucial to determine the actual PAC concentrations to which the organisms are exposed in the leachate. The experiments were carried out on a coking plant soil leachate stored in PS and glass bottles. Test conditions were determined based on the bioassay conditions i.e. 48 h, at 25°C in the dark for rotifers and 72 h, at 22°C under light for algae. Leachates were collected every 24 h for PS containers and at the end of the experiment for glass bottles. Results showed that the container material has a strong impact on the measured PAC concentrations in solution, especially for PAHs. PS containers induced a strong decrease in PAH concentrations under both test conditions and affect mostly high molecular weight compounds, most likely by sorption phenomenon. This decrease was not observed with glass container. Additionally, a decrease in low molecular weight compound concentrations was observed with both materials under algae test conditions which may result from PAH photodegradation Consequently, The PAH’s concentrations to which organisms are exposed during ecotoxicity tests are expected to be much lower than expected based on PAH levels in fresh leachate. These results pointed out the need to circumvent this experimental biais in order to calculate reliable ecotoxicity parameters. This may be achieved through an adjustment of test protocol to the physico-chemical properties and reactivity of studied molecules, or a calculation of theorical concentrations based on kinetic parameters of the process involved.
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Dates et versions

hal-04271344 , version 1 (06-11-2023)

Identifiants

  • HAL Id : hal-04271344 , version 1

Citer

Imane Aabbar, Catherine Lorgeoux, Pierre Faure, C. Cossu-Leguille, Céline Simon, et al.. Role of container materials on polycyclic aromatic compound (PAC) concentrations in aqueous phase: implication for aquatic ecotoxicity determination. SETAC EUROPE 33RD ANNUAL MEETING, Apr 2023, Dublin, Ireland. ⟨hal-04271344⟩
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