In this study, expressions for the interior stress fields of a spherical inclusion with uniform eigenstrain embedded in an anisotropic bi-material featuring a planar interface are derived. These expressions involve surface integrals of the imaginary term of the first derivative of the Green tensor in an anisotropic bi-material which are well-suited for standard numerical integrations. Specific formulations are provided for cases where the inclusion belongs to either the same material or both materials. Additionally, expressions are presented for the equivalent Eshelby tensor. The interior stress fields and variations in elastic strain energy are computed using cubic elastic constants of Cu. Various inclusion positions relative to the interface, eigenstrain forms, and crystallographic orientations are considered. Notably, stress variations within the inclusion are not necessarily monotonous. For instances involving dilatational eigenstrain, the elastic strain energy variation may exhibit multiple extrema. The global minimum and maximum consistently occur when the inclusion spans both materials. In the context of symmetrical tilt boundaries, energy variations are perfectly symmetric, with a global minimum on the interface that decreases with the tilt angle. The significance of the observed energy variations for defects segregation is quantitatively assessed by comparing them with the interaction energy between an eigenstrain and a grain boundary stress field.