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Conference Papers Year : 2024

Development of a functionalized hydrogel as cell recruiting biomaterial for tissue-engineering

Abstract

Arterial bypass operations (> 600,000) performed by using vascular grafts every year worldwide is the most extensively studied surgical procedure for cardiovascular vascular diseases, the leading causes of mortality globally [1]. Autologous blood vessels as the preferred vascular substitutes have limited availability in patients with some primary diseases including hypercholesterolemia and diabetes mellitus [2]. Synthetic materials such as Dacron® and polytetrafluoroethylene are suitable for large-diameter (> 6 mm internal diameter) blood vessels in clinical applications but not for small ones (< 6 mm internal diameter) due to low patency caused by thrombogenicity under the reduced blood-flow velocity [3]. Thus, the appropriate design of tissue-engineered blood vessels using functionalized coatings able to promote cell recruitment for inner surface endothelialization of decellularized vascular grafts might solve this issue. First, collagen I, the main protein forming the vascular extracellular matrix, is used to form a heparinized hydrogel either with low molecular weight heparin or unfractionated heparin. These heparins are used for their antithrombotic properties and bioactive molecule carrying capacity as well as their help in tuning hydrogel physicochemical properties. These heparinized hydrogels were evaluated for their physicochemical properties (kinetic of formation, heparin content detection, hydrogel spreading, viscosity, stability), their hemocompatibility (albumin adhesion), and their cytocompatibility (endothelial cells growth). Heparin incorporation in collagen hydrogels result in softer hydrogel formation and contact angle reduction without any impact on albumin absorption and cell viability compared with non-heparinized collagen hydrogel. Heparin can be retained in the hydrogels at least seven days after cell seeding, which provides the possibility of long-term antithrombotic properties. The developed coating will be either covered by cells seeded before graft implantation or implanted without cell covering to recruit host circulating cells present in the bloodstream.

Domains

Biomaterials
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Dates and versions

hal-04587074 , version 1 (24-05-2024)

Identifiers

  • HAL Id : hal-04587074 , version 1

Cite

Meiling Wu, Anne Sapin-Minet, Loïc Stefan, Julien Perrin, Isabelle Fries, et al.. Development of a functionalized hydrogel as cell recruiting biomaterial for tissue-engineering. Biomaterials and Biofabrication Conference – 2024, May 2024, Nancy, France. ⟨hal-04587074⟩
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