Harvesting extracellular matrix of wharton's jelly by extraction or decellularization process: a promising material for cardiovascular tissue engineering - Université de Lorraine Access content directly
Conference Papers Year : 2024

Harvesting extracellular matrix of wharton's jelly by extraction or decellularization process: a promising material for cardiovascular tissue engineering

Abstract

Abstract : Cardiovascular diseases such as coronary artery disease (CAD) are the leading cause of death worldwide. CAD most often results from the development of atherosclerosis, leading to total or partial occlusion of one or more coronary arteries. Depending on the case's severity the patient may require the replacement of one or more coronary arteries. To address this issue, vascular grafts of natural or synthetic origin have been successfully developed to restore blood flow. However, their applicability for the replacement of small diameter (< 6 mm) blood vessels is limited. Therefore, we are developing a 100 % human-derived vascular graft of small diameter using tissue-engineering based on the umbilical cord. This graft will be acellular to avoid immune reaction when implanted and to facilitate storage. To achieve this, we use decellularized umbilical artery as a scaffold that will be coated extracellular matrix (ECM) from Wharton's Jelly. ECM is harvested using either an extraction or a decellularization process found in the literature1,2. Our results indicate that the extraction process is much more efficient to collect different ECM components than decellularization process. Moreover, the addition of an ultrasound step3 to the extraction protocol allows for greater yield of collagens and proteins, increase the variety of angiogenesis factors retrieved and lower the residual quantity of DNA, compared to the decellularization process. Thus, this material could be of great interest for cardiovascular applications and in particular to coat small diameter vascular grafts to attain better performance and permeability. References 1. Kočí, Z. et al. Extracellular Matrix Hydrogel Derived from Human Umbilical Cord as a Scaffold for Neural Tissue Repair and Its Comparison with Extracellular Matrix from Porcine Tissues. Tissue Eng. Part C Methods 23, 333–345 (2017). 2. Kehtari, M. et al. Decellularized Wharton's jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells. J. Cell. Biochem. 120, 6683–6697 (2019). 3. Hussey, G. S. et al. Ultrasonic cavitation to prepare ECM hydrogels. Acta Biomater. 108, 77–86 (2020).

Domains

Bioengineering
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Dates and versions

hal-04587186 , version 1 (24-05-2024)

Identifiers

  • HAL Id : hal-04587186 , version 1

Cite

Nathan Wisniewski, Reine El Omar, Deborah Helle, Caroline Gaucher. Harvesting extracellular matrix of wharton's jelly by extraction or decellularization process: a promising material for cardiovascular tissue engineering. Biomat-MatSan 2024, Association française pour le développement des biomatériaux; Comission mixte MatSan (Matériaux pour la Santé), Jan 2024, Super Besse, France. ⟨hal-04587186⟩
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