Harvesting the Wharton’s Jelly Extracellular matrix using an ultrasound assisted Acetic Acid protocol to produce a material suitable for cardiovascular tissue engineering - Université de Lorraine Access content directly
Conference Papers Year : 2024

Harvesting the Wharton’s Jelly Extracellular matrix using an ultrasound assisted Acetic Acid protocol to produce a material suitable for cardiovascular tissue engineering

Abstract

Cardiovascular diseases are the leading cause of death worldwide according to the World Health Organization, with coronary artery disease (CAD) being the most common cardiovascular disease. To treat CAD, surgeons proceed with coronary artery bypass surgery using autologous saphenous vein or mammary artery as graft. When those graft are not available synthetic vascular grafts might be used with poor patency rates to replace those small diameter vessels (< 6 mm). Therefore, our team is developing an acellular small diameter vascular graft by tissue engineering based on human material coming exclusively from the umbilical cord. The absence of cells on the inner surface of the vascular graft requires a coating mimicking endothelial functions such as antithrombogenicity and facilitating graft recellularization to allow for a long-term patency. Wharton’s Jelly-derived extracellular matrix (WJ-ECM) extracted from the umbilical cord presents appropriate composition, being rich in collagen, glycosaminoglycans and growth factors, for a coating to support cell recruitment and colonization. Therefore, the first step was to develop a protocol to extract the ECM from the Wharton’s Jelly. To achieve this goal, we used an acetic acid extraction followed by an ultrasound treatment [1]. Our results indicate that this method allow for a low residual DNA quantity and that the addition of ultrasounds greatly increases collagen yield. Collagen type and structure are currently under investigation as well as the structure of the coating based on WJ-ECM. The addition of ultrasounds seems to have limited effects on the quantity and diversity of angiogenic factors retrieved. Thus, next steps are to evaluate coating ability to recruit cells and be colonized as well as to achieve coating anti thrombogenicity by heparinization before using it to cover the luminal surface of our graft to promote the graft’s integration within the host.

Domains

Bioengineering
No file

Dates and versions

hal-04587207 , version 1 (24-05-2024)

Identifiers

  • HAL Id : hal-04587207 , version 1

Cite

Nathan Wisniewski, Reine El Omar, Alexandra Kleinclauss, Gregory Francius, Deborah Helle, et al.. Harvesting the Wharton’s Jelly Extracellular matrix using an ultrasound assisted Acetic Acid protocol to produce a material suitable for cardiovascular tissue engineering. International Conference on Biomaterials and Biofabrication 2024, May 2024, Nancy, France. ⟨hal-04587207⟩
0 View
0 Download

Share

Gmail Mastodon Facebook X LinkedIn More