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Les protéines PilB, nDsbD et DsbE1 de Neisseria meningitidis : caractérisation enzymatique, fonctionnelle et structurale

Abstract : The Neisseria gonorrhoeae and Neisseria meningitidis species are human obligatory pathogenic bacteria, which acquired various defense mechanisms to detect and fight oxidative stress generated by mechanisms of host defense during infection. The periplasmic PilB protein, specific to these bacteria, would be part of such mechanisms and would be associated with their pathogenicity. PilB is composed of three domains: an N-terminal domain (Nter) with disulfide oxidoreductase activity, and central and C-terminal domains with Methionine sulfoxide reductase activity (Msr) of A and B class respectively. The study of isolated domains of PilB showed that the Nter domain selectively reduced MsrB domain. Moreover, this Nter domain presents a DsbE-fold. The DsbE are periplasmic disulfide oxidoreductases involved in the maturation of cytochrome c. In particular, Dr. Adeline Gand identified the DsbE1 from N. meningitidis during his PhD. During my PhD, the study of PilB proteins from N. meningitidis and Fusobacterium nucleatum allowed me to show that: 1) the selective reduction of Nter domain for the MsrB domain is not conserved, 2) the selective reduction of Nter domains observed on the isolated domains is not found in entire PilB, and 3) in all PilB, the MsrB domain reduction by Nter domain could be an intramolecular mechanism. Moreover, we studied the in vivo effect of the pilB gene deletion on the survival of a strain of N. meningitidis in the presence of oxidants. And, the N-terminal domain of DsbD protein (nDsbD) from N. meningitidis was identified as the reducing partner of periplasmic PilB and DsbE1 of N. meningitidis. Finally, the characterization of apocytochrome c reductase activity of DsbE1 N. meningitidis was complemented by in vitro and in vivo approaches in N. meningitidis
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Laure Selme-Roussel. Les protéines PilB, nDsbD et DsbE1 de Neisseria meningitidis : caractérisation enzymatique, fonctionnelle et structurale. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 2010. Français. ⟨NNT : 2010NAN10089⟩. ⟨tel-01746332⟩

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