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Application de la thérapie photodynamique sur des tumeurs de vessie induites chez le rat femelle Fischer 344

Abstract : The number of bladder cancers being in constant progression during the last few years, it is necessary to develop a representative bladder tumor model of human pathology in order to test the validity of new therapeutic methods as photochemotherapy. That's why we created a new bladder tumor model in the female Fischer 344 rat. After performing a local mechanical urothelial denudation, syngenic bladder tumor cells were instilled by uretral way. Development of induced tumors was followed by echography and their nature determined by histopathology. These are infiltrating transitional cell carcinomas with a constant and single localization at the bottom of the bladder and in the axis of uretra. In situ fluorescence spectroscopy, with a miniaturized optic fibers captor, has not allowed to distinguish strictly autofluorescence spectra of the normal bladder and the tumors at the wavelenght used. This non-invasive method has also been used to follow the biodistribution of a photosensitizer (PpIX) after administration by general (intraperitoneal) or local (instillation) route of its precursor (5-ALA) and to determine the maximal tumor/normal bladder ratios. These maximal ratios have been estimated : 2.85 ± 1.2 3h30 after intraperitoneal administration and 3.96 ± 1.04 after bladder instillation during 4h. These studies were confirmed by fluorescence microscopy, method which also allowed to specify the localization of PpIX according to the time and the 5-ALA administration route used. Finally, we carried out in situ photodynamic treatment of rat bladder tumors with a miniaturized frontal light distributor for the time tumor/normal bladder ratios were maximal. Therapeutic efficacy was defined by histopathological analysis. It was variable according to the infiltrating grade of the tumors but seemed to be independent with the 5-ALA administration route used.
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Submitted on : Thursday, March 29, 2018 - 10:49:43 AM
Last modification on : Friday, September 10, 2021 - 1:04:03 PM

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  • HAL Id : tel-01747026, version 1


Jean-François Bisson. Application de la thérapie photodynamique sur des tumeurs de vessie induites chez le rat femelle Fischer 344. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2000. Français. ⟨NNT : 2000NAN10290⟩. ⟨tel-01747026⟩



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