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Étude de l'échange d'ions modulé électriquement : application du couplage échange d'ions-électrodialyse à la séparation de biomolécules

Abstract : The present work aims to study the coupling of ion exchange and electrodialysis. This study is applied to the separation of biomolecules. One objective is to reduce the generation of saline wastewater produced by the ion exchange steps used conventionally in bioseparations. One approach has led to the design of architecture with a cyclic mode in 3 steps to purify some families of peptides without using a buffer pH or generate wastes. The experimental device consists of an electrodialysis cell in which are introduced anion exchange resins. The three steps are: loading of biomolecules on the resin initially in the carbonate form, elution with a solution of carbon dioxide dissolved in water, electroregeneration of the resin in its original form leading simultaneously to the regeneration of the carbonic acid solution. Using a modelling of the electroregeneration step, simulations can improve the understanding of coupled processes as the ion exchange equilibria, the equilibria in solution, the electromigration. A second approach has then been to study the possibilities of controlling the pH by electrochemical means to limit the use of buffers. The dissociation of water, leading to the formation of protons and hydroxyl ions, has been particularly studied by accounting the properties of contacts called « bipolar » as a result of an electric field. It was established that the choice of resin type and the current density can modify the pH. However this work must be pursued through research of architectures and operating procedures that deliver appropriate buffer capacity
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Wei Lu. Étude de l'échange d'ions modulé électriquement : application du couplage échange d'ions-électrodialyse à la séparation de biomolécules. Autre. Institut National Polytechnique de Lorraine, 2010. Français. ⟨NNT : 2010INPL027N⟩. ⟨tel-01748797⟩

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