Étude expérimentale et modélisation de la cinétique d'adhésion de particules colloïdales d'intérêt biologique aux interfaces solide/liquide

Abstract : aThis dissertation deals with the adhesion kinetics of synthetic colloidal particles and biological cells at a solid surface after sedimentation in a liquid. The experiments were carried out by means of a sedimentation cell observed with an optical microscope connected to a camera allowing to take images of the adsorbing surface at a constant frequency. An image analysis algorithm, capable of counting without ambiguity the particles adhering to the surface, was elaborated to determine precisely the kinetics of the irreversible adhesion of the particles on the adsorbing surface. Four types of synthetic particles, differing either by their diameters or by their densities, were used. The relative effect of the sedimentation under the influence of the gravity with respect to the Brownian diffusion is quantified by the dimensionless parameter R*. The measured kinetics were compared to the predictions of various models) this confrontation reveals the validity of the ballistic deposition model (where an infinitely intense gravity is assumed) for particles characterised by a high value of R*. As the value of R* decreases, the adhesion kinetics deviates from that predicted by this model. An original approach, which accounts;for the diffusion in a gravitational field of finite intensity and the formation of a multilayer, leads to a better reproduction of the experimental kinetics. The asymptotic domain, hence the jamming limit coverage, are also influenced by the mutual electrostatic repulsion of the particles. The methodology developed for the synthetic particles has been applied to the study of the deposition of polymorphonuclear leukocytes (PMN). It comes out from the observations that their irreversible adhesion kinetics is practically identical to that of synthetic particles characterised by the same value of R*.
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Philippe Carl. Étude expérimentale et modélisation de la cinétique d'adhésion de particules colloïdales d'intérêt biologique aux interfaces solide/liquide. Sciences agricoles. Institut National Polytechnique de Lorraine, 1999. Français. ⟨NNT : 1999INPL065N⟩. ⟨tel-01749902⟩

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