Synthèse et accumulation d'alcaloïdes tropaniques chez Datura innoxia Mill. cultivé en hydroponie : analyse des effets de l'environnement biotique et abiotique : essais de mise en place d'une nouvelle technologie de production

Abstract : Datura innoxia Mill., is a solanaceous plant synthetizing tropane alkaloids. It bas been studied to evaluate its potential when cultivated in soil-less conditions. As compared to classical culture conditions in soil, we could demonstrate that hydropony favoured growth and secondary metabolites accumulation within the biomass. Different biotic or abiotic treatments such as - 1) feeding the plants with tropane alkaloid precursors or AIA, 2) inoculation with Agrobacterium rhizogenes, 3) optimisation of light exposure or 4) stress treatments such as ozone exposure - can lead to hyoscyamine and scopolamine overproduction. These compounds are shown to be naturally exuded from roots into the nutrient solutions used for plant growth. Permeabilisation treatments using Tween20, temperature stress or sonication can drastically enhance such metabolites release. Coupled to trapping technology based on chromatographical . techniques, hydropony is shown to be a very promising tool for producmg plant metabolItes of interest.
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http://docnum.univ-lorraine.fr/prive/INPL_T_2005_TRAN_THI_LE_MINH.pdf
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Submitted on : Thursday, March 29, 2018 - 12:35:42 PM
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Thi Le Minh Tran. Synthèse et accumulation d'alcaloïdes tropaniques chez Datura innoxia Mill. cultivé en hydroponie : analyse des effets de l'environnement biotique et abiotique : essais de mise en place d'une nouvelle technologie de production. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2005. Français. ⟨NNT : 2005INPL042N⟩. ⟨tel-01750003⟩

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