Skip to Main content Skip to Navigation
Theses

Réponses adaptatives des communautés bactériennes telluriques aux métaux et métalloïdes : liens avec la disponibilité des polluants métalliques dans les sols

Abstract : Arsenic is a widespread toxic metalloid which is a major issue of public health. Its presence in the environment is naturally due to the geochemical background, i.e. the weathering of parent material and volcanic eruptions, but the main contamination sources are anthropogenic activities such as mining and metalworking industry. We describe a real-time PCR assay for the quantitative detection of arsB and ACR3(1) arsenite transporter gene families, two ubiquitous and key determinants of arsenic resistance in prokaryotes. The aioA gene encoding the large subunit of arsenite-oxidase was monitored in parallel. This study aimed to determine whether diversity, abundance and expression of these arsenite efflux pumps could serve as suitable biomarkers of metalloid stress and provide means to assess the impact of contamination on soil bacterial communities. The assay was applied in batch growth experiments using a wasteland soil bacterial community as an inoculum to investigate the effect of increasing arsenic concentrations on genes and transcripts abundances. To confirm previous results, further studies on the abundance and expression of arsB and ACR3(1) in indigenous soil bacterial communities exposed to different levels of arsenic over various time periods have helped to gain a better understanding of how these genes contribute to the adaptation of the communities to arsenic stress and their role in shaping the community structure and diversity. On the other hand, metal transporting PIB-type ATPases are critical components of bacterial resistance to cadmium, zinc and lead. We therefore designed degenerate PCR primers targeting PIB-type ATPases and tested their specificity on reference strains, metal-resistant soil isolates and soil metagenomic DNA
Complete list of metadata

Cited literature [219 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01750344
Contributor : Thèses Ul <>
Submitted on : Monday, September 21, 2020 - 11:55:58 AM
Last modification on : Friday, February 26, 2021 - 3:12:04 PM
Long-term archiving on: : Friday, December 4, 2020 - 5:54:32 PM

File

DDOC_T_2013_0164_POIREL.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01750344, version 1

Citation

Jessica Poirel. Réponses adaptatives des communautés bactériennes telluriques aux métaux et métalloïdes : liens avec la disponibilité des polluants métalliques dans les sols. Biochimie, Biologie Moléculaire. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0164⟩. ⟨tel-01750344⟩

Share

Metrics

Record views

114

Files downloads

119