Effet de la nature des biomatériaux sur la différenciation des cellules souches mésenchymateuses

Abstract : In tissue engineering, biomaterials, cells and the induction of cell differentiation are factors to be studied. The aim of this study is to know the effect of biomaterials composition and mechanical properties on the differentiation of mesenchymal stem cells from bone marrow. At first, we studied the effect of a protein biomaterial (collagen type I) supplemented with hydroxyaptatite (HAP) particles. We found that the addition of HAP improves the mechanical properties of the biomaterial and conditione cell differentiation towards osteoarticular lineages. In a second step, we studied the effect of biomaterial composed of alginate supplemented with hyaluronic acid or HAP particles, using an experimental design to select suitable matrices for biological study based on their mechanical properties. We found that the components of this biomaterial have an effect on elasticity of the latter and the differentiation of mesenchymal stem cells. In conclusion, this study shows that mesenchymal stem cells are sensitive to the composition of the biomaterial and its mechanical properties
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Fatima Ezzahra Laydi. Effet de la nature des biomatériaux sur la différenciation des cellules souches mésenchymateuses. Médecine humaine et pathologie. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0165⟩. ⟨tel-01750345⟩

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