Influence des précurseurs de la glycosylation sur la macrohétérogénéité de l'IFN-g produit en bioréacteur par des cellules CHO

Abstract : The aim of this work was the study of intracellular factors responsible for the macroheterogeneity of recombinant human IFN-gamma (IFN) glycosylation produced in bioreactor during batch and fed-batch cultures of Chinese Hamster Ovary (CHO) cells. Analysis of oligosaccharide precursors revealed that the structure of the lipid-linked oligosaccharides was constant over the batch process. On the contrary, the hypothesis of a quantitative limitation of the glycan structures could not be excluded. Moreover, the decline in intracellular nucleotide triphosphates and adenylate energy charge could be linked to the decrease in IFN glycosylation. This is supported by an unchanged pattern of IFN glycosylation when the cells displayed a high adenylate energy charge in a rich culture medium
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Nadine Kochanowski. Influence des précurseurs de la glycosylation sur la macrohétérogénéité de l'IFN-g produit en bioréacteur par des cellules CHO. Autre. Institut National Polytechnique de Lorraine, 2005. Français. ⟨NNT : 2005INPL082N⟩. ⟨tel-01752504⟩

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