Effet de l'environnement sur la croissance et l'accumulation de métabolites secondaires chez Datura innoxia Mill. cultivé en conditions hors sol ; impact des facteurs biotiques et abiotiques

Abstract : Datura innoxia Mill. a plant species belonging to Solanaceous family, produces hyoscyamine and scopolamine as two main tropane alkaloids. It has been studied in the present work for the development of hydroponic based secondary metabolites bioproduction. More specifically, biotic and abiotic environmental factors have been analysed in order to improve growth and alkaloid accumulation in plant tissues. Oxygen concentration in the nutrient solution is proved to be important for both biomass and compound production in temporary immersion culture conditions occur as one optimum. Temperature has also a major effect, directly and most probably through oxygen solubility and root needs. We showed that artificial light may positively complete natural one but the kind of spectrum must be chosen carefully. From this point of view, orange light from High Pressure Natrium Vapour light showed better results than classical white light. Nitrogen (NO3-15mM) and pH (5 to 6) management may lead to culture optimization. We also show that well chosen wild Agrobacterium rhizogenes strains may be added to the hydroponic nutrient solution in order to improve growth and alkaloid bioproduction. This result is due to the multi occurrence of genetic transformation events. Our results lead to a first classification for impacts of biotic and abiotic factors. It opens new window for plant milking technology industrial development
Complete list of metadatas

Cited literature [229 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01753048
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 2:05:33 PM
Last modification on : Tuesday, April 10, 2018 - 1:28:04 AM

File

2008_VU_T_D.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01753048, version 1

Collections

Citation

Thi Dao Vu. Effet de l'environnement sur la croissance et l'accumulation de métabolites secondaires chez Datura innoxia Mill. cultivé en conditions hors sol ; impact des facteurs biotiques et abiotiques. Sciences agricoles. Institut National Polytechnique de Lorraine, 2008. Français. ⟨NNT : 2008INPL029N⟩. ⟨tel-01753048⟩

Share

Metrics

Record views

84

Files downloads

199