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Encapsulation of Lactobacillus rhamnosus GG into hybrid alginate-silica microparticles

Abstract : One way to reestablish the microbiota equilibrium is to administrate a functional food containing probiotic cells. To insure protection of the living matter during the food processing and the gastrointestinal passage, encapsulation is essential. Herein we propose silica-based hybrid carriers as new probiotic delivery systems. Lactobacillus rhamnosus GG (LGG) was chosen as a model probiotic bacteria and alginate as prebiotic polymer. Two types of carriers were prepared either by emulsification or by electrospraying: (i) hybrid beads of 10-30 µm in which the bacteria was in direct contact with a mixture of alginate and silica and (ii) core-shell beads of 200-600 µm in which the bacteria are first embedded in an aqueous core of alginate, then coated with a silica shell. The viability of LGG was efficiently maintained only in core-shell particles, in which LGG was not directly exposed to silica. Those core-shell prototype carriers allowed the protection of LGG during storage in beer or apple juice, and during the passage through the gastrointestinal tract. Additionally the LGG released in the colon outcompete other members of the Lactobacilli community and it was able to thrive within a fecal ecosystem. This study offers thus a proof of concept for the potential use of hybrid silica/biopolymer systems in oral delivery of probiotic bacteria
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Submitted on : Wednesday, January 15, 2020 - 3:03:17 PM
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Haffner Fernanda Bianca. Encapsulation of Lactobacillus rhamnosus GG into hybrid alginate-silica microparticles. Chemical Sciences. Université de Lorraine, 2017. English. ⟨NNT : 2017LORR0079⟩. ⟨tel-01759292⟩

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