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Validation de l'utilisation des algues cryopréservées en écotoxicologie

Abstract : Using three algal species (Chlorella vulgaris, Selenastrum capricornutum and Scenedesmus subspicatus), different intrinsic and extrinsic factors have been studied (age, culture condition, cryoprotective treatment, freezing method, final temperature and storage duration). It emerges from this study that for each algal species will correspond a specific cryopreservation procedure. The use of complete factorial designs shows that : the three studied factors contribute to the viability of cells after freezing and all the three factors are in interaction. Tested with three metals (cadmium, copper and chromium) and an organic compound (atrazine), the cryopreserved algae are statistically more sensitive than fresh algae. For cadmium (chloride), for example, 72 H-IC50's are respectively 43,5 and 31.8 ppb for fresh and cryopreserved algae. The storage for three months at - 80°C has no effect on the viability of the cryopreserved samples and the variation of IC50's doesn't exceed 8.7%. The use of a filtration microplate allows the realization of a semi-static algal test with cryopreserved algae. This constitutes an important step in the making-up of a transportable, standardized, sensitive and more representative algal test
Mots-clés : Algues Cryotechnique
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Submitted on : Tuesday, April 24, 2018 - 3:50:49 PM
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  • HAL Id : tel-01776048, version 1



Ali Benhra. Validation de l'utilisation des algues cryopréservées en écotoxicologie. Ecotoxicologie. Université Paul Verlaine - Metz, 1994. Français. ⟨NNT : 1994METZ016S⟩. ⟨tel-01776048⟩



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