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Theses

Développement d’un réseau trophique microbien standardisé pour l’évaluation de l’impact des contaminants sur les écosystèmes dulçaquicoles

Abstract : In the framework of risk assessment, more environmentally realistic ecotoxicity tests taking complexity of ecosystems into account must be developed. Despite the importance of microorganisms in ecosystems and in their associated functional processes, this compartment is not enough taken into account in ecotoxicity assessment. The main objective of this thesis was the development of a model microbial food web to evaluate impact of contaminants on freshwater ecosystems. This network is composed of rotifers (Brachionus calyciflorus, primary and secondary consumers) that feed on both algae (Chlorella vulgaris, primary producer) and protozoa (Tetrahymena pyriformis, bacterivorous ciliate). A nitrifying microbial consortium produced in the laboratory associated with protozoa constitute a microbial loop that ensure trophic links between bacteria and metazoans. Organisms of the microbial network are in interaction by trophic relationships (prey-predator) and/or by functions they ensure within the ecosystem (oxygen production, primary production, nutrient recycling). The system can evolve into 4 ml over at least 28 days without medium renewing and, for example can allow to analyze the resilience of the system over a long period of time. In order to better evaluate different effects of pollutants on the food web, various analytical methods have been adapted and tested on this system. Among them, monitoring rotifer population dynamics was found to be a suitable parameter to evaluate the response of the miniaturized ecosystem to contaminants in a simple and fast way.
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https://hal.univ-lorraine.fr/tel-03184406
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Submitted on : Monday, March 29, 2021 - 2:26:05 PM
Last modification on : Tuesday, March 30, 2021 - 3:39:06 AM

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  • HAL Id : tel-03184406, version 1

Citation

Anne Vicente. Développement d’un réseau trophique microbien standardisé pour l’évaluation de l’impact des contaminants sur les écosystèmes dulçaquicoles. Biochimie, Biologie Moléculaire. Université de Lorraine, 2019. Français. ⟨NNT : 2019LORR0355⟩. ⟨tel-03184406⟩

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