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Screening and Purification of Metal Chelating Peptides in Hydrolysates for their Antioxidant Properties.

Abstract : Reactive Oxygen Species (ROS) induced oxidation Ageing and various pathologies in human related to oxidative stress, in particular inflammatory bowel diseases with iron anemia complication Degradation of consumption products (food, cosmetic matrix) Living organisms developped their own antioxidant defenses against these ROS Imbalance Oxidative stress Importance to discover natural antioxidants and evaluation of their bioactivity An interdisciplinary approach Some results SPR using a M 2+-chip:-a miniaturized approach of IMAC-type affinity chromatography-screening of the most interesting sources of metal-chelating peptides. Positive correlations evidenced between SPR screening and metal chelation tests using UV-Vis spectrometry on five sources of peptides. The affinity parameters are determined by SPR on some synthetic model peptides and then used to predict the separation of peptides on IMAC chromatographic column. Novel chromatography-MS method currently developed:-Screening of metal-chelating peptides sequences in a given complex mixture-Methodology successfully setup on a mixture of synthetic peptides-Further tests on real hydrolysates Identified peptides currently assessed for their antioxidant activity by chemical metal chelation and by in cellulo tests. Potential health application-Treatment of ferriprive anemia related to MICI, or inversely to hematochromatosis-Anchoring of these chelating peptides on nanoparticles for bioimaging or therapeutic applications. Metal-chelating peptides obtained after protein hydrolysis-Antioxidant properties related to the inhibition of Fenton reaction, responsible of ROS production-Used in pharmaceutics, foods and cosmetic Novel strategy for the screening and purification of the peptides to target their antioxidant properties. Novel approach developed in order to save time. Currently developed methods for the screening and separation of metal-chelating peptides by Surface Plasmon Resonance (SPR) by mass spectrometry (MS) : identification of the peptide(s) of interest present in the complex mixture Determination of metal chelation capacity using UV-visible spectrophotometry
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Contributor : Laetitia CANABADY-ROCHELLE Connect in order to contact the contributor
Submitted on : Friday, October 20, 2017 - 12:33:37 PM
Last modification on : Friday, March 4, 2022 - 2:33:31 PM
Long-term archiving on: : Sunday, January 21, 2018 - 12:12:23 PM


Canabady Rochelle-ISANH2016-LC...
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  • HAL Id : hal-01391695, version 1


Laetitia Canabady-Rochelle, Cédric Paris, Katalin Selmeczi, Caroline Gaucher, Igor Clarot, et al.. Screening and Purification of Metal Chelating Peptides in Hydrolysates for their Antioxidant Properties.. ISANH 2016, 2016, Paris, France. ⟨hal-01391695⟩



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