Chlordecone (Kepone) (CLD) is a highly persistent pesticide formerly used in French West Indies. Nowadays high levels of this pesticide are still found in soils which represent a subsequent source of contamination for outdoor-reared animals. In that context, sequestering matrices as activated carbons (ACs) or biochars are believed to efficiently decrease CLD transfer to animals. The present study intends to test using 2 distinct in vitro tests prior an in vivo assay the respective efficiency of several biochars and ACs to limit CLD transfer to animals. The Te-PBET and the ISO/DIS 16 751 availability part A protocols were used. In each test amended soils were prepared from a control one (SS) by adding 2% (mass basis) of one of the ACs or biochar. A selection of interesting matrices was realized prior the in vivo part of the protocol. For this final step piglets were exposed by alimentary route to contaminated soils. Only treatment groups exposed through amended soil with ACs presented significant decreases CLD availability, bioaccessibility (< 8%). Similar results were found using both in vitro assays. At last, concentrations of CLD in piglets liver and adipose tissue were found significantly lower after exposition to an AC amended soil (p< 0.001). This decrease was particularly high for a coconut shell activated carbon where relative bioavailability was found lower than 3% for both tissues. Finally, a positive correlation was found between environmental availability, bioaccessibility tests and in vivo results. This study leads to conclude that (i) AC introduced in CLD contaminated soil should strongly reduce CLD availability; bioaccessibility and bioavailability (ii) Tested biochars showed no reduction of transfert (iii) availability and bioaccessibility tests could be useful screening tests in order to select the appropriate biochar or AC.