Conception d'une nouvelle tête d'impression pour la réalisation de biopuces à l'ADN

Abstract : This work is focused on the design and fabrication of a new print head for biological sample deposition to elaborate DNA biochips. Biochips allow to analyse and to detect thousands of DNA samples (or proteins) in few hours. Biochips fabrication consists in arranging biological samples in form of spots on a solid substrate forming a microarray. Among current fabrications techniques, pin microarrayer is a simple and versatile recognized process. This process is based on direct contact between thin pins and the biochip support. The deposition is made through capillary forces. We propose two mechanical solutions for the improvement of the production performances in term of production time, flexibility and precision, in correspondence with the evolution of biological analysis methods. The first consists in compliant mechanism design for pin guiding. It makes it possible to offer an accurate positioning of each print pin and increases touching force control during spot deposition operation. Low space is available for each flexible guiding and we propose a new flexible structure. The second solution is selecting pins at each printing operation by up-and-down movement of pins. Proposed print head has 1536 pins. Operations of printing are decreased and vertical displacements of pins are reduced productivity is significantly increased. Reliability and flexibility are significantly improved. Shape memory alloy microactuators are developed for pin selection
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https://hal.univ-lorraine.fr/tel-01748972
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Manuel Vitasse. Conception d'une nouvelle tête d'impression pour la réalisation de biopuces à l'ADN. Médecine humaine et pathologie. Université Paul Verlaine - Metz, 2007. Français. ⟨NNT : 2007METZ006S⟩. ⟨tel-01748972⟩

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