Impact de l'agitation et de l'aération sur la réponse physiologique de Streptomyces pristinaespiralis DSMZ 40338 lors de sa culture en bioréacteurs mécaniquement agité et gazosiphon

Abstract : Previous results performed in Erlenmeyer flasks have shown that the hydrodynamics, characterized by power dissipation per unit of volume (P/V) and volumetric oxygen mass transfer coefficient (kLa), impacted the production of pristinamycins by Streptomyces pristinaespiralis (Mehmood, 2011). The aim of this work is then to study the influence of these two parameters in a stirred tank bioreactor (STR), widely used in industry, and in an airlift bioreactor. This last bioreactor has been designed specifically for this work. In a first part, the hydrodynamic environment was described in STR and the fluid flows were simulated by computational fluid dynamics (CFD). Using the same hydrodynamic conditions in STR and in airlift bioreactor than in flasks, the process performance (bacterial growth and pristinamycin production) were always lower in STR and airlift bioreactor. This demonstrates that P / V, kLa and also maximum dissipation were not pertinent scale-up criteria for the pristinamycin production from flask to STR or airlift bioreactor. On the contrary, the determined performances seemed to be related to the changes in bacterial morphology (presence and size of pellets) and to the physiology of the cells inside these structures. Surprisingly, during this work, the initiation of the pristinamycin production occurred almost always during the growth phase of S. pristinaespiralis. This phenomenon was probably due to the conjunction of several parameters which remain to identify
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Laial Haj Husein. Impact de l'agitation et de l'aération sur la réponse physiologique de Streptomyces pristinaespiralis DSMZ 40338 lors de sa culture en bioréacteurs mécaniquement agité et gazosiphon. Alimentation et Nutrition. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0199⟩. ⟨tel-01750452⟩

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