Skip to Main content Skip to Navigation
Theses

Écotoxicité de la deltaméthrine et du malathion sur différentes souches de "Daphnia magna" (Crustacea, Cladocera): apport de la protéomique dans la recherche de nouvelles cibles cellulaires

Abstract : The aim of this work was to evaluate different ecotoxic effects of deltamethrin and malathion on three strains of the cladoceran Daphnia magna. Two of the three strains belong to the same clone A (strains 1 and 3), which allowed to observe differences in inter-and intra-clonal sensitivity. The acute ecotoxicity tests (48h) showed for both deltamethrin and malathion, that one strain is more sensitive than strain 2, itself more sensitive than strain 3. Chronic tests (21days) showed that the common criterion mainly affected by both pesticides is the number of neonates per adult. These tests confirmed the sensitivity of the strain 1. Deltamethrin and malathion are embryotoxic as daphnid exposure to high concentrations of deltamethrin and malathion induced malformations. For these effects, strain 1 is also more sensitive than strain 2, itself more sensitive than strain 3. We also observed the appearance of males, but only in the offspring of the strain 1 exposed to deltamethrin. The determination of the specific activity of acetylcholinesterase (AChE), for both insecticides, showed a reversal of the order of susceptibility testing. Thus, as a biomarker of exposure and effect, AChE can also be considered as a biomarker of susceptibility. Proteomic analysis revealed several cellular targets of deltamethrin in strain 1 compared to strain 2. It confirms the greater sensitivity of strain 1 compared to strain 2. All these results point out that the genetic factor is a source of variation and highlight the opportunities offered by proteomic analysis
Complete list of metadata

https://hal.univ-lorraine.fr/tel-01751110
Contributor : Thèses Ul Connect in order to contact the contributor
Submitted on : Thursday, March 29, 2018 - 1:06:10 PM
Last modification on : Tuesday, October 19, 2021 - 7:01:19 PM

File

DDOC_T_2013_0343_TOUMI.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01751110, version 1

Citation

Héla Toumi. Écotoxicité de la deltaméthrine et du malathion sur différentes souches de "Daphnia magna" (Crustacea, Cladocera): apport de la protéomique dans la recherche de nouvelles cibles cellulaires. Biochimie, Biologie Moléculaire. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0343⟩. ⟨tel-01751110⟩

Share

Metrics

Record views

261

Files downloads

3143