Mise au point, optimisation et extrapolation de nouveaux procédés d'agitation et d'aération aux échelles 2L, 900L et 10m3 pour les souches D. Hansenii, s. Carnosus et P. Candidum

Abstract : The objectives of this thesis are, on one hand, to develop an efficient system of agitation in terms of oxygen transfer and shear stress, suited for the fermentation of yeasts, bacteria and filamentous fungi, on the other hand, to develop a process of spore production in a liquid medium. This new process will be used instead of the solid culture in Roux flasks used by DSM at the beginning of the PhD program. In a first step, we have selected “Elephant Ears” impellers as stirrers; its effectiveness of oxygen transfer was characterized in a broad range of stirring speeds and airflow rates. The data obtained were modeled and a predictive tool for KLa and process extrapolation was developed. The extrapolation is based on the non-geometrical similarity and the coefficient of oxygen transfer (KLa) was kept constant between scales. The effectiveness of the new stirrer was validated in a pilot-scale in the presence of the cultures of bacteria, yeasts and filamentous fungi. Besides the studies on the stirrer, a process in a liquid medium of filamentous fungi was developed. Thus, the fermentation medium and the operating conditions of each steps of the fermentation (germination, vegetative growth, and sporulation) were optimized. This new process makes possible to obtain up to 200.106 cells per milliliter of culture on both laboratory and pilot scale (900L). Finally, the data gathered on the pilot scale was used to extrapolate this process to the industrial-scale of 10m3
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Hugues Canteri. Mise au point, optimisation et extrapolation de nouveaux procédés d'agitation et d'aération aux échelles 2L, 900L et 10m3 pour les souches D. Hansenii, s. Carnosus et P. Candidum. Alimentation et Nutrition. Université de Lorraine, 2014. Français. ⟨NNT : 2014LORR0332⟩. ⟨tel-01751557⟩

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