Skip to Main content Skip to Navigation
Theses

Spectroscopie bimodale en diffusion élastique et autofluorescence résolue spatialement : instrumentation, modélisation des interactions lumière-tissus et application à la caractérisation de tissus biologiques ex vivo et in vivo pour la détection de cancers

Abstract : This research activity aims at developing and validating a multimodal spectroscopy method in elastic scattering and autofluorescence to characterize biological tissues in vitro and in vivo. It is articulated in four axes. At first, instrumentation is considered with the development, the engineering and the experimental characterization of a fibers bimodal, multi-points spectrometry system allowing the acquisition of spectra in vivo (variable distances, fast acquisition). Secondly, the optical properties of tissues are modelled with the development and the experimental validation on phantoms of a photons propagation simulation algorithm in turbids media and multi-fluorescent. Thirdly, an experimental study has been conducted ex vivo on fresh and cryopreserved arterial rings. It confirms the complementarity of spectroscopic measurements in elastic scattering and autofluorescence, and validates the method of multi-modality spectroscopy and the simulation of photons propagation algorithm. Results have well proved a correlation between rheological and optical properties. Finally, one second experimental study in vivo related to a pre-clinical tumoral model of bladder has been carried out. It highlights a significant difference in diffuse reflectance and/or autofluorescence and/or intrinsic fluorescence between healthy, inflammatory and tumoral tissues, on the basis of specific wavelength. The results of not supervised classification show that the combination of various spectroscopic approaches increases the reliability of the diagnosis
Complete list of metadatas

Cited literature [227 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01752957
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 2:02:52 PM
Last modification on : Friday, June 12, 2020 - 5:58:02 PM

File

2007_PERY_E.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01752957, version 1

Collections

Citation

Emilie Pery. Spectroscopie bimodale en diffusion élastique et autofluorescence résolue spatialement : instrumentation, modélisation des interactions lumière-tissus et application à la caractérisation de tissus biologiques ex vivo et in vivo pour la détection de cancers. Autre [cond-mat.other]. Institut National Polytechnique de Lorraine, 2007. Français. ⟨NNT : 2007INPL079N⟩. ⟨tel-01752957⟩

Share

Metrics

Record views

103

Files downloads

578