Etude expérimentale de la réinfection par le protozoaire parasite toxoplasma gondii

Abstract : The Protozoon "Toxoplasma gondii" can infect all warm blooded vertebrates and induce an acute infection followed by a chronic stage that persists as tissue cysts. The primary infection is usually believed to protect against any further infection by the parasite. However, this belief has never been formally proven, because there were no convenient tools to distinguish between two coinfection strains. Our project aimed at investigate this matter experimentally. We have therefore developed a new model using a transgenic strain of "T. gondii" expressing the "E. coli" LacZ gene (strain Pru[bêta]gal) which can be easily distinguished from wild strains in situ. We have then performed a series of infections and reinfections asociating the Pru[bêta]gal strain with several others strains belonging to the two major cystogenic "T gondii" genotypes. We have demonstrated that a reinfection was possible, but only with a reinfecting strain of a different genotype from the primary strain. We have also studied the early stages of parasitic development and shown that, when the protection to reinfection operates, it does so during the first hours of interaction between the parasite and the intestinal mucosa of the host. Last, we have combined our model with a microsatellite approach to study the kinetics of development of the protection to reinfection. We have shown that it was complete 7 days after primary infection.
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Anne Dao Thuc Trinh. Etude expérimentale de la réinfection par le protozoaire parasite toxoplasma gondii. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10294⟩. ⟨tel-01754333⟩

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