Étude de la phytochimie de 12 plantes de la région Lorraine en fonction de la granulométrie de poudres superfines

Abstract : This project focuses on the study of 12 plants coming from Lorraine region. The study aims to improve the exploitation of local flora using a dry extraction process. A milling and a sieving process up to fine particles of plant material is used to concentrate the bioactive compounds in the resulting powders. The powders were classified according to the particle size which were ranging from 20 µm to 500 µm. The phytochemical properties of each particle size fraction are compared to non-sieved plant parts. These activities are linked to secondary metabolites including polyphenols and terpene derivatives, which are characterized and quantified by LC-MS and GC-MS analyses. It was developed in this work a new validation technique of differential separation depending on the particle size of the powders compared with extracts of non-sieved plant parts. The quantification of chemical compound classes was done by UV-Visible methods and their identification was conducted using LC-PDA/MS and GC-MS characterization technics. The evaluations of the antioxidant activity and anti-acetylcholinesterase activity is carried out in vitro according to the particle sizes. This study demonstrates the usefulness of this new differential extraction process of bioactive compounds from vegetal matrices. The Enhancing of the concentration of the active products is observed according to the particle sizes. In silico modeling study of anti-acetylcholinesterase activity is employed to highlight the interactions between the active sites and some anticipated active compounds in the extract
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https://hal.univ-lorraine.fr/tel-01935491
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  • HAL Id : tel-01935491, version 1

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Ali Zaiter. Étude de la phytochimie de 12 plantes de la région Lorraine en fonction de la granulométrie de poudres superfines. Agronomie. Université de Lorraine, 2017. Français. ⟨NNT : 2017LORR0012⟩. ⟨tel-01935491⟩

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